KRAS[G12C](GDP load) His&Avi Tag Protein, Human Antibody Elution Fixation: Cells were fully resuspended

Fixation: Cells were fully resuspended by adding an appropriate amount of 4% paraformaldehyde (prepared in PBS) and fixed at 4 ° C for 30 min

it folds in the endoplasmic reticulum with a 12 kDa β2-microglobulin light chain to create a peptide-binding groove that preferentially accommodates 8–10-mer peptides bearing hydrophobic C-termini

4) prepared by diluting Reagent D or other dialysis buffers can be used

2、Sample addition:

is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses

KRAS[G12C](GDP load) His&Avi Tag Protein, Human Antibody Elution Fixation: Cells were fully resuspendedProduct Specification Species Human Synonyms GTPase KRas,KRAS2,RASK2 Accession P01116 2 Amino Acid Sequence M1 K169,with N terminal 8x His tag,C terminal Avi Expression System E. coli Molecular Weight 23. 6 kDa Purity >85% by SDS PAGE Conjugation Unconjugated Tag Avi Tag, His Tag Physical Appearance Liquid Storage Buffer 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7. 5 Stability & Storage Stable for 12 months upon stored at 80 from the date of