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and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against mitochondrial uncoupling protein 3 (UCP3)
25uL of Matrigel per well was spread in A 24-well cell culture plate
Eukaryotic elongation factor 2 (eEF2) is a crucial regulator of protein synthesis
but not all astrocytes express S100B
HEMCAM Accession P43121 Amino Acid Sequence Val24-Gly559
Rat LC3 ELISA Kit Size:96T and HRP conjugate are sequentiallyProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
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