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need not operation this step)
If the plate reader does not have a wavelength of 570nm or 630nm
3、Add biotinylated antibody:
Glennie: Immunotherapy Targeting Inhibitory Fcγ Receptor IIB (CD32b) in the Mouse Is Limited by Monoclonal Antibody Consumption and Receptor Internalization
Source Mouse Synonym Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor ELISA Kit Detection Type Double antibody sandwich method Composition Name 9 6 T match set remark Pre-coating 96 Well plate 8 Hole ×12 Strip without Standard 2 branch Dilute as per instructions
Human CKLF1 ELISA Kit Size:96T need not operation this step)Product Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20